GAZI UNIVERSITY INFORMATION PACKAGE - 2019 ACADEMIC YEAR

COURSE DESCRIPTION
APPLIED MOLECULAR BIOLOGICAL TECHNIQUES/TBG-5101
Course Title: APPLIED MOLECULAR BIOLOGICAL TECHNIQUES
Credits 2 ECTS 6
Semester 1 Compulsory/Elective Elective
COURSE INFO
 -- LANGUAGE OF INSTRUCTION
  Turkish
 -- NAME OF LECTURER(S)
  Prof. Dr. Ece KONAC, Assoc.Prof. Dr. Hacer Ilke ONEN, Assoc.Prof. Dr. Atiye Seda YAR SAGLAM
 -- WEB SITE(S) OF LECTURER(S)
  http://www.websitem.gazi.edu.tr/site/ecem, http://www.websitem.gazi.edu.tr/site/hionen, http://www.websitem.gazi.edu.tr/site/sedayar
 -- EMAIL(S) OF LECTURER(S)
  ecem@gazi.edu.tr, hionen@gazi.edu.tr, atiyeseda@yahoo.com
 -- LEARNING OUTCOMES OF THE COURSE UNIT
To be able to practice DNA extraction steps from cultured cells
To be able to practice RNA extraction steps from cultured cells
To be able to practice protein extraction steps from cultured cells
To be able to check the rate of growth (or cell division) of the population





 -- MODE OF DELIVERY
  The mode of delivery of this course is face to face
 -- PREREQUISITES AND CO-REQUISITES
  TBG-5100 Moleküler Biyolojik Teknikler
 -- RECOMMENDED OPTIONAL PROGRAMME COMPONENTS
  TBG-5100 Moleküler Biyolojik Teknikler
 --COURSE CONTENT
1. Week  Medium preparation for cell line
2. Week  Resuscitation of frozen cell lines
3. Week  Measuring the rate of growth (or cell division) of the population
4. Week  Measuring the rate of growth (or cell division) of the population
5. Week  Sub-culturing
6. Week  To determine the number of cell culture-amplified cells
7. Week  To determine cell viability
8. Week  DNA extraction from cultured cells
9. Week  Sub-culturing
10. Week  RNA extraction from cultured cells
11. Week  Sub-culturing
12. Week  Protein extraction from cultured cells
13. Week  Determination of the amount and purity of DNA, RNA and proteins
14. Week  Cryopreservation and storage of cell lines
15. Week  Report presentation
16. Week  Practice exam
 -- RECOMMENDED OR REQUIRED READING
  Güler Temizkan, Nazlı Arda, Temel ve İleri Moleküler Biyoloji Yöntemleri-Genomik ve Proteomik Analizler, Nobel Tıp Kitabevleri, 2018.
 -- PLANNED LEARNING ACTIVITIES AND TEACHING METHODS
  Lecture, Question & Answer, Demonstration, Drill - Practise
 -- WORK PLACEMENT(S)
  Medical Biology and Genetics Laboratories
 -- ASSESSMENT METHODS AND CRITERIA
 
Quantity
Percentage
 Mid-terms
0
0
 Assignment
1
10
 Exercises
0
0
 Projects
0
0
 Practice
1
30
 Quiz
0
0
 Contribution of In-term Studies to Overall Grade  
40
 Contribution of Final Examination to Overall Grade  
60
 -- WORKLOAD
 Efficiency  Total Week Count  Weekly Duration (in hour)  Total Workload in Semester
 Theoretical Study Hours of Course Per Week
0
 Practising Hours of Course Per Week
13
2
26
 Reading
13
2
26
 Searching in Internet and Library
13
2
26
 Designing and Applying Materials
0
0
0
 Preparing Reports
7
4
28
 Preparing Presentation
0
0
0
 Presentation
1
1
1
 Mid-Term and Studying for Mid-Term
0
 Final and Studying for Final
15
3
45
 Other
0
 TOTAL WORKLOAD: 
152
 TOTAL WORKLOAD / 25: 
6.08
 ECTS: 
6
 -- COURSE'S CONTRIBUTION TO PROGRAM
NO
 PROGRAM LEARNING OUTCOMES
1
2
3
4
5
1X
2
3
4X
5
6X
7X
8
9
10