GAZI UNIVERSITY INFORMATION PACKAGE - 2019 ACADEMIC YEAR

COURSE DESCRIPTION
Applied Genetics/TBG-6031
Course Title: Applied Genetics
Credits 2 ECTS 10
Semester 1 Compulsory/Elective Elective
COURSE INFO
 -- LANGUAGE OF INSTRUCTION
  Turkish
 -- NAME OF LECTURER(S)
  Prof. Ece KONAC, Assoc. Prof. Hacer Ilke ONEN, Assoc. Prof. Atiye Seda YAR SAGLAM
 -- WEB SITE(S) OF LECTURER(S)
  http://www.websitem.gazi.edu.tr/site/ecem, http://www.websitem.gazi.edu.tr/site/hionen, http://www.websitem.gazi.edu.tr/site/sedayar
 -- EMAIL(S) OF LECTURER(S)
  ecem@gazi.edu.tr, hionen@gazi.edu.tr,sedayar@gazi.edu.tr
 -- LEARNING OUTCOMES OF THE COURSE UNIT
To be able to prepare karyotype and to interpret pedigree analysis
To be able isolate DNA from blood or tissue
To be able isolate RNA from blood or tissue
To be able to quantify and purity of DNA and RNA
To be able to practise different PCR reaction types and applications
To be able isolate protein from blood or tissue
To be able to practise methods for genotyping
To be able to prepare agarose gels
To be able to practise protein detection and analysis methods
 -- MODE OF DELIVERY
  The mode of delivery of this course is face to face and practical training
 -- PREREQUISITES AND CO-REQUISITES
  There is no prerequisite or co-requisite for this course
 -- RECOMMENDED OPTIONAL PROGRAMME COMPONENTS
  There is no recommended optional programme component for this course.
 --COURSE CONTENT
1. Week  Preparation of karyotype and pedigree analysis
2. Week  DNA isolation from blood
3. Week  DNA isolation from tissue
4. Week  RNA isolation from blood
5. Week  RNA isolation from tissue
6. Week  Determination of DNA and RNA Concentration and Purity
7. Week  Different types of PCR techniques and its applications
8. Week  Presentation assignment
9. Week  Midterm
10. Week  Application of PCR primer design
11. Week  SNP analysis by RFLP method
12. Week  Preparation of agarose gels
13. Week  Protein isolation from blood
14. Week  Protein isolation from tissue
15. Week  Protein detection and analysis methods
16. Week  Final Exam
 -- RECOMMENDED OR REQUIRED READING
  Güler Temizkan, Nazlı Arda, Temel ve İleri Moleküler Biyoloji Yöntemleri-Genomik ve Proteomik Analizler, Nobel Tıp Kitabevleri, 2018. Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Keith Roberts, Peter Walter. Molecular Biology of the Cell (Ed.6th), Garland Science, USA, 2014.
 -- PLANNED LEARNING ACTIVITIES AND TEACHING METHODS
  Lecture, Question & Answer, Demonstration, Drill - Practise
 -- WORK PLACEMENT(S)
  Medical Biology and Genetics Laboratories
 -- ASSESSMENT METHODS AND CRITERIA
 
Quantity
Percentage
 Mid-terms
1
40
 Assignment
1
10
 Exercises
0
0
 Projects
0
0
 Practice
0
0
 Quiz
0
0
 Contribution of In-term Studies to Overall Grade  
50
 Contribution of Final Examination to Overall Grade  
50
 -- WORKLOAD
 Efficiency  Total Week Count  Weekly Duration (in hour)  Total Workload in Semester
 Theoretical Study Hours of Course Per Week
14
1
14
 Practising Hours of Course Per Week
14
1
14
 Reading
14
4
56
 Searching in Internet and Library
14
4
56
 Designing and Applying Materials
0
0
0
 Preparing Reports
0
0
0
 Preparing Presentation
7
5
35
 Presentation
1
2
2
 Mid-Term and Studying for Mid-Term
8
5
40
 Final and Studying for Final
6
6
36
 Other
0
0
0
 TOTAL WORKLOAD: 
253
 TOTAL WORKLOAD / 25: 
10.12
 ECTS: 
10
 -- COURSE'S CONTRIBUTION TO PROGRAM
NO
 PROGRAM LEARNING OUTCOMES
1
2
3
4
5
1X
2X
3X
4X
5X
6X
7X
8
9
10