GAZI UNIVERSITY INFORMATION PACKAGE - 2019 ACADEMIC YEAR

COURSE DESCRIPTION
MOLECULAR BIOLOGICAL TECHNIQUES/TBG-5100
Course Title: MOLECULAR BIOLOGICAL TECHNIQUES
Credits 2 ECTS 6
Semester 1 Compulsory/Elective Compulsory
COURSE INFO
 -- LANGUAGE OF INSTRUCTION
  Turkish
 -- NAME OF LECTURER(S)
  Prof. Dr. Ece KONAC, Prof. Dr. Ozdemir Serhat GUROCAK, Assoc.Prof. Dr. Hacer Ilke ONEN, Assoc.Prof. Dr. Atiye Seda YAR SAGLAM
 -- WEB SITE(S) OF LECTURER(S)
  http://www.websitem.gazi.edu.tr/site/ecem, http://www.websitem.gazi.edu.tr/site/hionen, http://www.websitem.gazi.edu.tr/site/sedayar
 -- EMAIL(S) OF LECTURER(S)
  ecem@gazi.edu.tr, sgurocakmd@yahoo.com, hionen@gazi.edu.tr, atiyeseda@yahoo.com
 -- LEARNING OUTCOMES OF THE COURSE UNIT
To be able to explain equipment and devices in the cell culture laboratory
To be able to explain the basic requirements of cell culture
To be able to explain DNA extraction steps from cultured cells
To be able to explain RNA extraction steps from cultured cells
To be able to explain protein extraction steps from cultured cells
 -- MODE OF DELIVERY
  The mode of delivery of this course is face to face
 -- PREREQUISITES AND CO-REQUISITES
  There is no prerequisite or co-requisite for this course
 -- RECOMMENDED OPTIONAL PROGRAMME COMPONENTS
  There is no recommended optional programme component for this course.
 --COURSE CONTENT
1. Week  An Introduction to Cell Culture
2. Week  Equipment and devices in the cell culture laboratory
3. Week  Sourcing of Cell Lines
4. Week  Preparation of cell growth medium
5. Week  Resuscitation of frozen cell lines
6. Week  Sub-culturing
7. Week  Methods for determining the number of cell culture-amplified cells
8. Week  Presentation assignment
9. Week  Midterm
10. Week  Methods for determining cell viability
11. Week  DNA extraction methods from cultured cells
12. Week  RNA extraction methods from cultured cells
13. Week  Protein extraction methods from cultured cells
14. Week  DNA RNA and determination of the amount and purity of proteins
15. Week  Cryopreservation and storage of cell lines
16. Week  Final exam
 -- RECOMMENDED OR REQUIRED READING
   Güler Temizkan, Nazlı Arda, Temel ve İleri Moleküler Biyoloji Yöntemleri-Genomik ve Proteomik Analizler, Nobel Tıp Kitabevleri, 2018.
 -- PLANNED LEARNING ACTIVITIES AND TEACHING METHODS
  Lecture, Question & Answer
 -- WORK PLACEMENT(S)
  N/A
 -- ASSESSMENT METHODS AND CRITERIA
 
Quantity
Percentage
 Mid-terms
1
30
 Assignment
1
10
 Exercises
0
0
 Projects
0
0
 Practice
0
0
 Quiz
0
0
 Contribution of In-term Studies to Overall Grade  
40
 Contribution of Final Examination to Overall Grade  
60
 -- WORKLOAD
 Efficiency  Total Week Count  Weekly Duration (in hour)  Total Workload in Semester
 Theoretical Study Hours of Course Per Week
13
2
26
 Practising Hours of Course Per Week
0
0
0
 Reading
13
2
26
 Searching in Internet and Library
13
2
26
 Designing and Applying Materials
0
0
0
 Preparing Reports
0
0
0
 Preparing Presentation
7
2
14
 Presentation
1
1
1
 Mid-Term and Studying for Mid-Term
8
4
32
 Final and Studying for Final
6
4
24
 Other
2
1
2
 TOTAL WORKLOAD: 
151
 TOTAL WORKLOAD / 25: 
6.04
 ECTS: 
6
 -- COURSE'S CONTRIBUTION TO PROGRAM
NO
 PROGRAM LEARNING OUTCOMES
1
2
3
4
5
1X
2
3
4X
5
6X
7X
8X
9X
10X