GAZI UNIVERSITY INFORMATION PACKAGE - 2019 ACADEMIC YEAR

COURSE DESCRIPTION
Practical recombinant DNA technology/MİK-4041
Course Title: Practical recombinant DNA technology
Credits 2 ECTS 6
Course Semester 1 Type of The Course Elective
COURSE INFORMATION
 -- (CATALOG CONTENT)
 -- (TEXTBOOK)
 -- (SUPPLEMENTARY TEXTBOOK)
 -- (PREREQUISITES AND CO-REQUISITES)
 -- LANGUAGE OF INSTRUCTION
  Turkish
 -- COURSE OBJECTIVES
 -- COURSE LEARNING OUTCOMES
to learn an essential information about recombinant DNA technology and molecular biology methods
To be able to perform basic recombinant DNA technology practices
To learn the basic knowledge about Molecular Biothecnology
To learn strategies of cloning
To perform gene libraries and to apply cloning of cDNA
To be able to cut and join the DNA molecules
To select and screen the recombinants
To able to express the clonned DNA molecules in Escherichia coli
To be able to apply DNA sequencing and new generation sequencing systems
To be able to perform recombinant DNA applications

 -- MODE OF DELIVERY
  The mode of delivery of this course is Face to face
 --WEEKLY SCHEDULE
1. Week  Basic technics
2. Week  Plasmids as cloning vectors
3. Week  Bacteriophages
4. Week  Cosmid vectors
5. Week  Strategies of cloning
6. Week  Gene libraries and cloning of cDNA
7. Week  Cutting and joining of DNA molecules
8. Week  Mid-term examination
9. Week  Selection and screening of recombinants
10. Week  Expression of clonned DNA molecules in Escherichia coli
11. Week  Analysis of DNA sequencing and new generation sequencing systems
12. Week  Replacement of the genes
13. Week  Cloning in bacteriae apart from E.coli
14. Week  Cloning in Saccharomyces cerevisiae
15. Week  Applications of recombinant DNA
16. Week  x
 -- TEACHING and LEARNING METHODS
 -- ASSESSMENT CRITERIA
 
Quantity
Total Weighting (%)
 Midterm Exams
1
40
 Assignment
4
30
 Application
8
30
 Projects
0
0
 Practice
8
0
 Quiz
0
0
 Percent of In-term Studies  
60
 Percentage of Final Exam to Total Score  
40
 -- WORKLOAD
 Activity  Total Number of Weeks  Duration (weekly hour)  Total Period Work Load
 Weekly Theoretical Course Hours
0
 Weekly Tutorial Hours
14
2
28
 Reading Tasks
0
 Searching in Internet and Library
14
1
14
 Material Design and Implementation
14
2
28
 Report Preparing
14
1
14
 Preparing a Presentation
14
2
28
 Presentation
0
 Midterm Exam and Preperation for Midterm Exam
14
1
14
 Final Exam and Preperation for Final Exam
14
1
14
 Other (should be emphasized)
0
 TOTAL WORKLOAD: 
140
 TOTAL WORKLOAD / 25: 
5.6
 Course Credit (ECTS): 
6
 -- COURSE'S CONTRIBUTION TO PROGRAM
NO
 PROGRAM LEARNING OUTCOMES
1
2
3
4
5
1X
2X
3X
4X
5X
6
7
8
9X
10X
 -- NAME OF LECTURER(S)
   (Prof.Dr.Meltem Yalınay Çırak)
 -- WEB SITE(S) OF LECTURER(S)
   (http://websitem.gazi.edu.tr/site/meltemyc)
 -- EMAIL(S) OF LECTURER(S)
   (meltemyc@gmail.com)