GAZI UNIVERSITY INFORMATION PACKAGE - 2019 ACADEMIC YEAR

COURSE DESCRIPTION
PRACTICES IN MOLECULAR BIOLOGY TECHNIQUES IN TOXICOLOGY/FTO-5050
Course Title: PRACTICES IN MOLECULAR BIOLOGY TECHNIQUES IN TOXICOLOGY
Credits 3 ECTS 7.5
Course Semester 1 Type of The Course Elective
COURSE INFORMATION
 -- (CATALOG CONTENT)
 -- (TEXTBOOK)
 -- (SUPPLEMENTARY TEXTBOOK)
 -- (PREREQUISITES AND CO-REQUISITES)
 -- LANGUAGE OF INSTRUCTION
  Turkish and Eng
 -- COURSE OBJECTIVES
 -- COURSE LEARNING OUTCOMES
To learn application of molecular biology techniques
The ability to effectively use laboratory skills acquired in toxicological researches
The ability to perform comprehensive toxicological data analysis, analyze new ideas, synthesize and evaluate
The demonstrate excellence and innovation in problem-solving skills, interpreting intellectual level, and high level oral and written communication sk
The ability to evaluate and apply the health risks of drugs and other chemical substances in human-.Chemical risk assessment skills
Gain the ability to understand the mechanisms of action of carcinogenic and mutagenic agents
To learn the principles of application and evaluation of toxic effects of chemical agents in in vivo and in vitro test systems
Gain the ability to understand the role of target organs in the toxic effects of drugs and other chemicals
Ability to monitor and investigate adverse effects of drugs
The ability to analyze the molecular and cellular effects of chemicals and radiation on male and female reproductive systems and developing embryo / f
 -- MODE OF DELIVERY
  The mode of delivery of this course is Face to face
 --WEEKLY SCHEDULE
1. Week   The basic molecular biology techniques in Toxicology
2. Week   DNA extraction from biological materials (blood, sperm and some tissues) I
3. Week   DNA extraction from biological materials (blood, sperm and some tissues) II
4. Week  The amplification of DNA samples by PCR (polymerase chain reaction)
5. Week  The principles of optimization of PCR conditions for newly diagnosed polymorphisms
6. Week   The preparation of gels for vertical and horizontal electrophoresis systems
7. Week   The loading of DNA to agarose gels, Electrophoresis
8. Week  8. Week : The loading of DNA to polyacrylamide gels, Electrophoresis
9. Week  The detection of SNPs by RFLP (Restriction Fragment Length Polymorphism) technique
10. Week   The application of multiple SNPs detection
11. Week   The determination of allele frequencies of SNPs in major drug metabolizing or DNA repair genes (GSTs, OGG1, NAT2.) by PCR-RFLP techniques
12. Week   The determination of allele frequencies of SNPs in susceptibility of specific disease (breast, lung, bladder cancer..) by PCR-RFLP techniques
13. Week   Evaluation of allele frequencies in both group (patient and healthy), calculation of HW Equilibrium
14. Week   The determination of the disease susceptibility
15. Week  
16. Week  
 -- TEACHING and LEARNING METHODS
 -- ASSESSMENT CRITERIA
 
Quantity
Total Weighting (%)
 Midterm Exams
1
25
 Assignment
1
25
 Application
0
0
 Projects
0
0
 Practice
0
0
 Quiz
0
0
 Percent of In-term Studies  
50
 Percentage of Final Exam to Total Score  
50
 -- WORKLOAD
 Activity  Total Number of Weeks  Duration (weekly hour)  Total Period Work Load
 Weekly Theoretical Course Hours
14
3
42
 Weekly Tutorial Hours
0
 Reading Tasks
2
10
20
 Searching in Internet and Library
2
10
20
 Material Design and Implementation
0
 Report Preparing
2
10
20
 Preparing a Presentation
2
10
20
 Presentation
2
9
18
 Midterm Exam and Preperation for Midterm Exam
1
18
18
 Final Exam and Preperation for Final Exam
1
30
30
 Other (should be emphasized)
0
0
 TOTAL WORKLOAD: 
188
 TOTAL WORKLOAD / 25: 
7.52
 Course Credit (ECTS): 
7.5
 -- COURSE'S CONTRIBUTION TO PROGRAM
NO
 PROGRAM LEARNING OUTCOMES
1
2
3
4
5
1X
2X
3X
4X
5X
6X
7X
8X
9X
10X
11X
12
13
14X
15
 -- NAME OF LECTURER(S)
   (Prof. Bensu KARAHALİL)
 -- WEB SITE(S) OF LECTURER(S)
   (http://www.websitem.gazi.edu.tr/site/bensu)
 -- EMAIL(S) OF LECTURER(S)
   (bensu@gazi.edu.tr)