GAZI UNIVERSITY INFORMATION PACKAGE - 2019 ACADEMIC YEAR

COURSE DESCRIPTION

TECHNIQUES USED IN BIOTECHNOLOGY/6151301

Course Title:	TECHNIQUES USED IN BIOTECHNOLOGY
Credits	3	ECTS	7.5
Course Semester	1	Type of The Course	Elective

COURSE INFORMATION

-- (CATALOG CONTENT)

-- (TEXTBOOK)

-- (SUPPLEMENTARY TEXTBOOK)

-- (PREREQUISITES AND CO-REQUISITES)

-- LANGUAGE OF INSTRUCTION

Turkish

-- COURSE OBJECTIVES

-- COURSE LEARNING OUTCOMES

Learning Biotechnological and Molecular Techniques

-- MODE OF DELIVERY

The mode of delivery of this course is Face to face

--WEEKLY SCHEDULE
1. Week	Biotechnological definitions
2. Week	Isolation of chromosomal DNA, RNA and plasmid
3. Week	Plant and mammalian DNA isolation, RNA / DNA purity and amount
4. Week	cDNA synthesis, cDNA and genomic DNA library
5. Week	Electrophoresis techniques (Paper, Agorose, SDS-PAGE, Capillary, Immunoelectrophoresis)
6. Week	Electrophoresis techniques (Pulse Field Gel Electrophoresis, 2D-PAGE)
7. Week	PCR using techniques and primary design, RAPD-PCR, AFLP (Amplified Fragment Lenght Polymorphism), Anchored PCR, Asymmetric PCR
8. Week	Homework presentations
9. Week	Touchdown PCR, RT PCR, inverse PCR, Multiplex Nested PCR techniques, Immuno PCR, Real Time PCR, PCR array
10. Week	Restriction endonuclease enzymes and their applications
11. Week	Vectors, Recombinant DNA technology
12. Week	Omics Technologies (genomics, proteomics, transcriptomics), Bioinformatics, NCBI and applications
13. Week	DNA sequence analysis (Sequence), New generation DNA sequence analysis
14. Week	Homework Presentations
15. Week	Final Examination
16. Week

-- TEACHING and LEARNING METHODS

-- ASSESSMENT CRITERIA

	Quantity	Total Weighting (%)
Midterm Exams	0	0
Assignment	2	40
Application	0	0
Projects	0	0
Practice	0	0
Quiz	0	0

Percent of In-term Studies		40
Percentage of Final Exam to Total Score		60

-- WORKLOAD

Activity	Total Number of Weeks	Duration (weekly hour)	Total Period Work Load
Weekly Theoretical Course Hours	14	3	42
Weekly Tutorial Hours			0
Reading Tasks	14	2	28
Searching in Internet and Library	14	3	42
Material Design and Implementation	0	0	0
Report Preparing	7	2	14
Preparing a Presentation	7	2	14
Presentation	2	3	6
Midterm Exam and Preperation for Midterm Exam	7	2	14
Final Exam and Preperation for Final Exam	14	2	28
Other (should be emphasized)			0
TOTAL WORKLOAD:			188
TOTAL WORKLOAD / 25:			7.52
Course Credit (ECTS):			7.5

-- COURSE'S CONTRIBUTION TO PROGRAM

-- NAME OF LECTURER(S)

(Prof. Dr. Belma ASLIM)

-- WEB SITE(S) OF LECTURER(S)

(websitem.gazi.edu.tr/baslim)

-- EMAIL(S) OF LECTURER(S)

(baslim@gazi.edu.tr)

Gazi University