GAZI UNIVERSITY INFORMATION PACKAGE - 2019 ACADEMIC YEAR

COURSE DESCRIPTION
Applied Molecular Biological Techniques I/TBG-1021
Course Title: Applied Molecular Biological Techniques I
Credits 3 ECTS 9
Semester 1 Compulsory/Elective Compulsory
COURSE INFO
 -- LANGUAGE OF INSTRUCTION
  Turkish
 -- NAME OF LECTURER(S)
   Prof. Ece KONAÇ, Prof. Abdullah EKMEKÇİ, Prof. Taner AKAR, Assoc.Prof. Hacer İlke ÖNEN, Assoc. Prof. Atiye Seda YAR SAĞLAM
 -- WEB SITE(S) OF LECTURER(S)
  http://websitem.gazi.edu.tr/site/ecem
 -- EMAIL(S) OF LECTURER(S)
  ecem@gazi.edu.tr
 -- LEARNING OUTCOMES OF THE COURSE UNIT
Learn the laboratory security
Learn to preparation of acidic and basic solutions
Learn to calculations of molarity and normality
Learn to preparation of solutions and calculations
To be able to prepare of buffer solution
Acquaring skills of DNA and RNA isolation
Learn to use Nanodrop for measuring DNA and RNA
Learn the PCR
Acquaring skills of agarose gel electrophoresis
Acquaring skills of loading samples on agorose gel
 -- MODE OF DELIVERY
  The mode of delivery of this course is Face to face.
 -- PREREQUISITES AND CO-REQUISITES
  There is no prerequisite or co-requisite for this course.
 -- RECOMMENDED OPTIONAL PROGRAMME COMPONENTS
   There is no recommended optional programme component for this course.
 --COURSE CONTENT
1. Week  Laboratory security
2. Week  Acidic and basic solutions
3. Week  Applied solution preparation
4. Week  DNA and RNA isolation
5. Week  DNA and RNA isolation
6. Week  DNA and RNA isolation
7. Week  Measure DNA and RNA
8. Week  PCR (Polymerase Chain Reaction) methods
9. Week  PCR (Polymerase Chain Reaction) methods
10. Week  PCR (Polymerase Chain Reaction) methods
11. Week  Agorose gel and electrophoresis
12. Week  Agorose gel and electrophoresis
13. Week  Agorose gel and electrophoresis
14. Week  Agorose gel and electrophoresis
15. Week  
16. Week  
 -- RECOMMENDED OR REQUIRED READING
  Basic Molecular Biology:Essential Techniques, Philappa D.Darbre Essential Molecular Biology, A Practical Approach Applied Molecular Biology Paperback –by Chao-Hung Lee
 -- PLANNED LEARNING ACTIVITIES AND TEACHING METHODS
   Demonstration, Drill - Practise
 -- WORK PLACEMENT(S)
  NA
 -- ASSESSMENT METHODS AND CRITERIA
 
Quantity
Percentage
 Mid-terms
0
0
 Assignment
1
40
 Exercises
1
60
 Projects
0
0
 Practice
0
0
 Quiz
0
0
 Contribution of In-term Studies to Overall Grade  
40
 Contribution of Final Examination to Overall Grade  
60
 -- WORKLOAD
 Efficiency  Total Week Count  Weekly Duration (in hour)  Total Workload in Semester
 Theoretical Study Hours of Course Per Week
14
1
14
 Practising Hours of Course Per Week
14
3
42
 Reading
0
 Searching in Internet and Library
14
2
28
 Designing and Applying Materials
14
3
42
 Preparing Reports
8
2
16
 Preparing Presentation
8
3
24
 Presentation
8
2
16
 Mid-Term and Studying for Mid-Term
0
 Final and Studying for Final
14
3
42
 Other
1
1
1
 TOTAL WORKLOAD: 
225
 TOTAL WORKLOAD / 25: 
9
 ECTS: 
9
 -- COURSE'S CONTRIBUTION TO PROGRAM
NO
 PROGRAM LEARNING OUTCOMES
1
2
3
4
5
1- Understanding of basic concepts in genetics ,application of genetic information in diagnosis and monitoring the course of human diseases,application of genetic technologies in medicine,advanced molecular genetics,cellular biology,molecular biology of human neoplastic diseases.X
2Learning structures of cell membranes and organelles in detail at the molecular level.X
3Learning basic rules of patterns of inheritance and pedigree analysis.X
4Knowing chemical and physical properties of DNA, RNA and proteins.X
5Becoming familiar with new instruments and technologies that are required to do an experimental research in the cell and molecular biology labs.X
6Learns the basic molecules which are responsible for the storage and duplication of genetic information and its transfer into proteins .X
7Gets acquainted with molecular biology focusing on gene transcription and protein-protein interactionsX
8Acquaring skills of DNA and RNA isolation, quantitating DNA or RNA, PCR, electrophoresis, RT-PCR, cell culture.X
9Understanding the roles of genetic and epigenetic mechanisms observed in genes in the process of evolution and transmit of hereditary features and disorders from one generation to another.X
10Learns the meaning of genetic code and its role in the relationship between the structure and function of organisms.X